Extraction of Fibrillar and Sarcoplasmic Proteins of Turkey Musclea

Abstract

SUMMARYProtein extraction and fractionation was carried out on samples of breast and leg muscles of turkey males 11–32 weeks old. Nitrogen determinations were made on the total, extractable, coagulable, actomyosin, myosin, and sarcoplasmic protein fractions. These components respectively contained 4.27, 2.57, 1.96, 0.53, 0.09, and 1.36 g nitrogen per 100 g of muscle (wet weight). Nitrogen values were higher for light muscle than for dark muscle.Muscle samples were taken from 21–36‐week‐old turkey males both before and after 48 hr at 5–10°C. Results showed a 4.9% decrease in total nitrogen and a 3.4% increase in non‐protein nitrogen.Amounts of protein fractions were compared between birds of three age groups. Birds within the 25–32‐week‐old group gave highest values for all fractions estimated with the exception of total nitrogen. Turkeys ranging in age from 21–26 weeks gave the highest values for this component. Extractable, coagulable, and nonprotein values increased with increasing age; the 11‐week‐old turkeys gave the lowest values.