Extraction of Campomanesia xanthocarpa fruit using supercritical CO2 and bioactivity assessments

Abstract

This study reports extraction of Campomanesia xanthocarpa fruit using supercritical CO2 and its chemical composition and biological activity analysis. The experiments were carried out at 15.0, 20.0 and 25.0MPa and 313.15, 333.15 and 353.15K. The maximum yield observed was 3.90wt% at 313.15K and 25MPa. The pressure had a significant and positive effect on extraction yields, whereas the temperature effect was significant and negative. Gas chromatography analysis showed that the main compounds identified were α-eudesmol, β-eudesmol, γ-eudesmol, caryophyllene (E), α-sabinene, β- sabinene, germacrene B, δ-cadinene, humulene and selina-3,7(11)-diene. The highest values of antioxidant activity were found for the extracts obtained at (313.15K and 25MPa) and at (353.15K and 15.0MPa), determined by the DPPH method and the phosphomolybdenum reduction method, respectively. The extract obtained at 353.15K and 25MPa presented the highest phenolic content (39.12mg GAE/g of extract). The extract obtained at 313.15K and 15MPa presented the maximum antibacterial activity against Staphylococcus aureus. The Gram-negative bacteria tested (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimuruim) and Listeria monocytogenes were completely resistant toward the C. xanthocarpa fruit extracts.