Extraction of bioactive compounds from Botryosphaeria dothidea using supercritical carbon dioxide and compressed liquefied petroleum gas

Abstract

This study presents extraction yields, kinetic curves, kinetic parameters, antioxidant capacity and composition of extract obtained from dry biomass of the fungus Botryosphaeria dothidea. The metabolites were produced by submerged fermentation using ultrasound as pretreatment. Thereafter, levels of pressure and temperature were evaluated for recovering the metabolites by supercritical CO2 (150, 200 and 250 bar; 40, 50 and 60 °C) and compressed liquefied petroleum gas (LPG) (15, 25 and 30 bar; 20, 30 and 40 °C). Ethanol was also used as cosolvent, which the mixture CO2 + 10 wt.% ethanol provided an extraction yield of 1.14 wt.%. When pure CO2 or LPG was used, the global yields did not exceed 0.86 wt.% and 0.94 wt.%, respectively. Fatty acids, esters, sterols and fatty acid methyl esters were some types of compounds recovered. Furthermore, the antioxidant capacity was measured, with the most promising result as 38.4 ± 3.1 μmol TEAC/μg extract.