Abstract
Spirulina platensis is a bioactive-rich microalga of great potential as a source of natural antioxidant. Natural antioxidants are increasing in demand, because it is considered as more safe than synthetic antioxidants. The objective of this study was to determine the effect of solvent on the potential of natural antioxidant from fresh S. platensis. The antioxidant compound was extracted using either acetone or ethyl acetate by reflux method. The yield of extractions were 1.86% and 3.07% for aceton and ethyl acetate, respectively. Fresh S. platensis extracts were considered as potential antioxidant source with IC50 values 65.89 ppm, and 76.36 ppm when using acetone and ethyl acetate, respectivetly, even though it were still lower compare to the IC50 of positive control quercetin i.e 21.64 ppm. Phytochemical test showed positive results of phenolic compound, triterpenoids, steroid, flavanoid, and saponin. aw value of Spirulina platensis was 0.61 and 0.81 for aceton and ethyl acetate, respectively. It was concluded that different solvent affected the antioxidant activity of S. platensis extract. Keywords: Antioxidants, aceton, ethyl acetate, fresh Spirulina platensis
Highlights
Spirulina platensis is a bioactive-rich microalga of great potential as a source
Natural antioxidants are increasing in demand
The objective of this study was to determine the effect of solvent on the potential
Summary
Bahan dan Alat Bahan yang digunakan dalam penelitian ini adalah mikroalga Spirulina platensis yang diambil dari kolam pembudidayaan di CV. Alat-alat yang digunakan dalam penelitian ini adalah Refluks (Pyrex iwaki), Sonikator (Branson 200), Sentrifuge (Table Top Centrifuge Plc 03 Series), Rotary evaporator (Buchi R 210), Spektrofotometer (Shimadzu). Prosedur Penelitian Proses ekstraksi ini adalah menggunakan metode refluks yang dikombinasi dengan sonikasi. Ekstraksi dilakukan dengan mempersiapkan sampel Spirulina platensis segar sebanyak 300 gram dimasukkan ke dalam 2 tabung erlenmeyer masing-masing 150 g, kemudian tabung 1 ditambahkan pelarut aseton dan tabung 2 ditambahkan pelarut etil asetat sebanyak 50 mL. Kedua sampel dimasukkan ke dalam sonikator untuk dilakukan proses sonikasi selama 1 jam dengan gelombang 40 Khz pada suhu ruang (28oC). Analisa Data Metode yang digunakan pada penelitian ini adalah metode experimental laboratories dengan analisa data secara deskriptif. Metode pengolahan data yang dilakukan adalah dengan cara membandingkan hasil uji dengan kontrol positif, kemudian dijelaskan dengan hasil kulitatif sebagai penguat yang mendukung data kuantitatif
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
