Extraction of Andrographis paniculata as Material for Developing Soap and Sanitizer

Abstract

The aim of this work to analyse quantitative study of chemical properties of different A. paniculata extracts obtained from different extraction methods which are methanol and ethanol extracts by maceration and Soxhlet extraction. This consists of antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC). This project also aims to study the chemical and physicochemical properties of the developed soap and sanitizer.in order to evaluate the quality of the products. The properties of original extracts were compared with the products in order to investigate the improvement of the antioxidant contents of the products upon addition of the A. paniculata extract to them. The antioxidant activity is evaluated using 2,2-diphenyl-1-picrylhdrazyl test. TPC is determined by using Folin-Ciocalteu calorimetric method where Folin-Ciocalteu reagent is used. TFC is quantified by following aluminium chloride calorimetric method. All the tests are quantified using spectrophotometric. Simple antimicrobial assay by hand printing method on agar plate media was also done to analyze the efficiency of the sanitizers against humans’ pathogens on hands. The results showed the A. paniculata macerated methanol extract exhibited highest antioxidant activity with 76.027% as well as the highest TPC and TFC with 0.222 mg GAE/ml and 7.500 mg QE/ml. The results also showed both products were able to retain similar antioxidant content, higher TPC and lower TFC to that indicated by the original plant extract used to prepare the products. The result showed the improvement of antioxidant contents of soap and sanitizer upon the addition of A. paniculata extracts. Therefore, A. paniculata extracts are potent natural ingredients for antioxidant properties purpose and can be used to replace the harmful synthetic ingredients on products formulations.