Abstract

The escalating demand for bioactive compounds from plants has grown substantially, primarily attributed to their potential therapeutic applications across diverse industries such as pharmaceuticals, nutraceuticals, and cosmetics. This comprehensive review examines the extraction methods employed for isolating bioactive compounds from plant sources, focusing on techniques that maximise yield, maintain compound integrity, and ensure sustainability. Conventional methods, including solvent extraction, are extensively discussed, encompassing well-established techniques such as maceration, Soxhlet extraction, and hydrodistillation. These methods have gained widespread usage due to their simplicity and cost-effectiveness. In addition to traditional approaches, this review explores novel methods such as ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), and supercritical fluid extraction (SFE). These emerging techniques are recognised for their capacity to enhance extraction efficiency and reduce processing times significantly. Factors influencing the extraction process and subsequent bioactive compound yield, such as solvent selection, particle size, and temperature, are thoroughly investigated. The review also scrutinises the ecological impact and economic considerations associated with each method, aligning with the growing emphasis on sustainable practices in plantbased compound extraction. Advancements in extraction technologies, including ionic liquids and enzyme-assisted extraction, are highlighted, emphasising their potential to improve specificity and selectivity in isolating bioactive compounds. The paramount importance of selecting the most suitable extraction method based on the chemical nature of the target compounds and the characteristics of the plant matrix is underscored throughout the discussion. In conclusion, this review provides a comprehensive synthesis of the current state of plant extraction methods for bioactive compounds. It meticulously delineates the advantages and limitations of conventional and emerging techniques. It offers valuable insights for researchers, practitioners, and industries aiming to optimise the extraction process for enhanced bioactive compound yields while prioritising environmental and economic sustainability

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