Extraction, Isolation and Identification of Luteolin Flavonoid from Vitex pseudonegundo leaves

Abstract

Objective: Using HPLC as a quantitative estimate technique, the optimal extraction solvent for luteolin from Vitex pseudo-negundo was identified. Methods: leaves V. pseudonegundo were gathered, washed, and dried. employeing methanol and ethanol as the extraction solvents (both with varied strengths), as well as the extraction techniques of maceration, reflux, and Soxhlet. The amount of luteolin in each sample extract was measured using a preparative HPLC technique Results: Methanol was the best solvent, and the best extraction method was the reflex extraction method, based on HPLC results Conclusion: According to the results of HPLC, reflex was the superior method for extracting luteolin from V. pseudonegundo, and the optimum solvent was methanol. thus, advising using this approach in industry and future study.