Abstract
The flavonoids contained in C. singueana aerial parts were extracted, identified and characterized. Sequential Soxhlet extracts were subjected to preliminary phytochemical screening and flavonoid quantification. The results showed that maximum yield of the flavonoid (8.435 mg/g) were obtained from DCM extract. The DCM extract was subjected to flash chromatography for isolation of flavonoid. Isolated flavonoid was characterized using various spectroscopic techniques. On the basis of chemical and spectral analyses structure was elucidated as 2-3, (4-dihydroxyphenyl)-3, 5, 7-trihydroxy-4H-chromen-4-one, a flavonoid. Keywords: Cassia Singueana, Flavonoids, Flash chromatography, Quercetin
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