Extraction, Isolation and Analysis of Chondroitin Sulphate from Chicken Shank by Spectrophotometric Method

Abstract

This study evaluated Chondroitin sulphate isolated from chicken shank cartilage in aspects such as sufficient quantities and requisite quality for feasible source of Chondroitin sulphate. Chondroitin sulphate has been widely used in treatment of osteoarthritis. Chondroitin sulphate not only reduces joint pain, but also inhibiting the disease progression. Chondroitin sulphate was obtained from cartilage of chicken shank, using enzyme (papain), acid (acetic acid), and heat. The cartilage obtained from chicken shank was hydrolysed using papain for 24h. Extraction was carried out using acetic acid and incubated at pH 4.5 and 37°C for 6h and 8h. The supernatan was treated with cetylpyridinium chloride (CPC), NaCl, methanol and ethanol. The precipitate obtained from extraction was dried in oven at 55°C.Chondroitin sulphate yield from 6h incubation was 16.30% and from 8h incubation was 36.35 % (dry weight). The FT-IR spectra of extracted Chondroitin sulphate was identical with Chondroitin sulphate standard (Fenchem Biotek Ltd). Overlay of the spectras showed characteristic absorption at the wave number 854.64 cm-1 for sample and 856.54 cm-1 for standard chondroitin sulphate. Quantitative analysis was performed using spectrophotometry. The precision was good with RSD <7.3% and the accuration was good with recovery of 82.48-108.88%.The detection limit and quantification limit were 0.15 ppm and 0.46 ppm. This study shows that chicken shank cartilage produces a source for isolation of chondroitin sulphate of 36.35% (dry weight) and purity of 87.87%.