Abstract
A DNA extraction method was developed that preferentially extracted extracellular DNA rather than intracellular DNA from forest litter. The method purposely avoided the use of harsh chemicals and physical disruption steps used in total DNA extraction to release DNA from cells. The detection limit of PCR, determined by spiking forest litter samples with a dilution series of Choristoneura fumiferana MNPVegt −/ lacZ + genomic DNA, was about 1 ng DNA or 6.85×10 6 target copies 0.5 g −1 moist forest litter or 0.14 g −1 dry forest litter. In this study, outdoor terrestrial microcosms, each spiked with 49.2 μg of genomic DNA (from the baculovirus CfMNPVegt −/ lacZ +), were exposed to summer conditions. A 530 bp DNA fragment from the genome of the baculovirus CfMNPVegt −/ lacZ + was detected in these microcosms for about 3 months. The DNA may have persisted for a longer period but was below the detection limit of the PCR analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
