Extraction, characterization and gelling behavior enhancement of pectins from the cladodes of Opuntia ficus indica

Abstract

Total Pectins Fraction (TPF) was extracted at room temperature from dried cladodes of Opuntia ficus indica. TPF is constituted of three pectic fractions WSP, CSP and ASP, which are made up of 66.6%, 44.3% and 81.1% (w/w) of galacturonic acid, respectively. The antioxidant ability of TPF increased with the concentration increasing. It scavenged hydroxyl radical by 90% and chelated 90% of ferrous ions at 5g/L. FTIR study was carried out. Strong characteristic absorption peaks at 1618cm−1 assigned to the vibration of COO– group of galacturonic acid. In the fingerprint region, we noticed three well-defined peaks at 1054, 1085, and 1154cm−1 characteristic of pectic polysaccharides. TPF are non-gelling pectins. The co-crosslinking of TPF with carrageenan was carried out and the gelling behavior was successfully improved. Thermo-sensitive hydrogel was obtained with 82% of TPF and 18% of carrageenan (w/w).