Extraction, characterization and antioxidant activity of polysaccharide from Pouteria campechiana seed

Abstract

In this study, the seed polysaccharides (PCSP) was ultrasonic-assisted extracted from Pouteria campechiana and optimized by response surface method (RSM). After separation and purification by DEAE-52 cellulose column and Sephadex G-75 glucan gel column, the pure polysaccharide component of PCSPa-1 was obtained, and its structure and antioxidant activity were analyzed. The results showed that the optimal parameters of PCSP with maximum yields (15.94%) were ultrasonic temperature of 79 °C, ultrasonic time of 69 min, and liquid to material ratio of 41 mL/g. The molecular weight of PCSPa-1 was 67900 Da. PCSPa-1 consisted of glucose and mannose with a molar ratio of 86.65:4.62, and the glycosidic bond mainly included →4)-α-d-Glc(1→ and →6)-α-d-Glc(1→. Scanning electron microscopy showed that PCSPa-1 was a strip structure with a smooth surface. In addition, PCSPa-1 had strong scavenging capacity to 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis- (3-ethylbenzthiaz oline-6-sulphonic acid) (ABTS), hydroxyl radical, and superoxide radical. Polysaccharides of Pouteria campechiana seeds could be exploited as a natural antioxidant.