Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii.

Abstract

Methods to determine the presence of a bacterial capsule typically involve the use of stains specific for capsule, the exclusion of a dye by the capsule or by electron microscopy. However, these procedures require equipment that may not be readily available to all researchers. Here we describe a method for extraction, visualization, and quantification of extracellular capsular polysaccharide from Acinetobacter baumannii with commonly used reagents and equipment.