Extraction and purification of oridonin from Rabdosia rubescens and its protective effect on intracerebral hemorrhage-induced brain injury in rats

Tie Li,Xueyan Wang,Kun Dong

doi:10.1590/fst.24921

Abstract

Oridonin was extracted and purified from Rabdosia rubescens and its protective effect on intracerebral hemorrhage (ICH)-induced brain injury in rats was investigated. The rats were divided into sham, model, low-dose oridonin, middle-dose oridonin and high-dose oridonin groups. The ICH-induced brain injury model was established in latter four groups. After modeling, the low-, middle- and high-dose oridonin groups were treated with 10, 20, and 40 mg/kg oridonin at 2h and 24 h after modeling, respectively. Results showed that, after 48 after modeling, compared with model group, in middle- and high-dose oridonin groups the modified Neurological Severity Score was significantly decreased (P < 0.05), the brain water content was significantly decreased (P < 0.05), the brain tissue tumor necrosis factor α, interleukin 1β, interleukin 6 and nuclear factor-kappa B (NF-κB) p65 protein levels were significantly decreased (P < 0.05), and the brain tissue erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) protein levels were significantly increased (P < 0.05). In conclusion, oridonin from Rabdosia rubescens can mitigate the ICH-induced brain injury in rats. The mechanism may be related to its reduction of inflammatory response and oxidative stress by inhibiting NF-κB inflammatory pathway and promoting Nrf2/HO-1 antioxidant pathway in brain tissues.

Highlights

Practical Application: This study has provided a reference for preparation of oridonin from Rabdosia rubescens and application of it to treat intracerebral hemorrhage-induced brain injury
Intracerebral hemorrhage (ICH) is one of the acute cerebrovascular diseases, which accounts for 10% to 20% of all strokes worldwide and is associated with high morbidity and mortality (Rindler et al, 2020)
More and more studies have shown that the inflammatory response and oxidative stress after hematoma formation are closely related to the ICH-induced brain injury and neurological dysfunction (Wasserman et al, 2007; Sugiyama et al, 2018)

Summary

Introduction

Intracerebral hemorrhage (ICH) is one of the acute cerebrovascular diseases, which accounts for 10% to 20% of all strokes worldwide and is associated with high morbidity and mortality (Rindler et al, 2020). The secondary brain injury caused by ICH is an important factor leading to the neurological damage (Belur et al, 2013). More and more studies have shown that the inflammatory response and oxidative stress after hematoma formation are closely related to the ICH-induced brain injury and neurological dysfunction (Wasserman et al, 2007; Sugiyama et al, 2018). The clinical treatment of ICH-induced brain injury includes supportive measures and surgery (Thiex and Tsirka, 2007; Holste et al, 2021). It is very important to find effective drugs to prevent and treat ICHinduced brain injury and the neurological dysfunction. Rabdosia rubescens belongs to the Lamiaceae plants. A diterpenoid compound, is the main active component of Rabdosia rubescens. The nuclear factor-kappa B (NF-κB) inflammatory pathway (Zhang et al, 2015) and nuclear factor erythroid 2-related factor 2/heme oxygenase 1

Methods

Results

Conclusion