Abstract

Indole acetic acid (IAA) produced from F. oxysporum (F2) was purified by several steps included extraction by cold ethyl acetate ; Column chromatography using silica gel and TLC chromatography . The pure indole acetic acid (IAA) which produce by F. oxysporum (IAA) was tested by ultraviolet spectra at (200-300)nm ; and appear that the maximum absorbance at 229nm , the high performance liquid chromatography (HPLC) used to test the purity of the indole acetic acid and the results showed one peak at appearance time 3.822 min

Highlights

  • ‫الرييقة )‪(TLC‬‬ ‫اتبعتتتتت ريقتتتتة وروموتو راويتتتتا ال بقتتتتة‬ ‫الرقيقتة ‪ TLC‬المولتووة متن قبتل ‪ Maor‬وجماعتتت‬ ‫[‪ ]15‬باستعمال الواح من الالمنيوم م ليتة ب بقتة متن‬ ‫مادة رصم السليوا ‪ Silica gel‬نوع ‪ G‬المجهزة من قبتل‬ ‫شتتروة ‪ ، Fluka‬وباس تتخدام محل تول الال تل المتوتتون‬ ‫متن (متاء لوحتول مويلتي ل ولورووتورم ) بنستبة

  • (1976): Ester of Indole-3 Acetic Acid from Aven.seed.Plant Physiol.58:60-67

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Summary

Introduction

‫الرييقة )‪(TLC‬‬ ‫اتبعتتتتت ريقتتتتة وروموتو راويتتتتا ال بقتتتتة‬ ‫الرقيقتة ‪ TLC‬المولتووة متن قبتل ‪ Maor‬وجماعتتت‬ ‫[‪ ]15‬باستعمال الواح من الالمنيوم م ليتة ب بقتة متن‬ ‫مادة رصم السليوا ‪ Silica gel‬نوع ‪ G‬المجهزة من قبتل‬ ‫شتتروة ‪ ، Fluka‬وباس تتخدام محل تول الال تل المتوتتون‬ ‫متن (متاء لوحتول مويلتي ل ولورووتورم ) بنستبة

Results
Conclusion
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