Abstract
Abstract: The study were conducted at Dilla University, College of Natural Sciences, Biological Sciences laboratories. Background: Ethiopia is a country with many ethnic groups, cultures and beliefs which in turn have contributed to the high diversity of traditional health care knowledge and practices of traditional medicine from local growth plants, animals and minerals for various physical and mental disorders of human and livestock population that passed from generation to generation for centuries. Medicinal plants contributors to pharmaceutical, agricultural and food industries in the world. The use of medicinal plants in the industrialized societies has been traced to extraction and development of several drugs used in order to heel some diseases having inhibiting effect against pathogenic microorganism. Objective: The main objective of this study was Extraction and Phytochemicals determination of traditional medicinal plants for anti microbial susceptibility test. Methodology: The extraction and identification of some phytochemicals crude compound which used for antimicrobial susceptibility test from plant sample such as Ocimum lamiifolium (OL), Croton maerosth (Cm) and Ruta chalepesis (RC) were conducted. Plant samples are collected, powdered using mortal and pistil and extracted using ethanol and some susceptibility tests were performed to identify some phytochemicals compound. Result: The main result of Antimicrobial activity test showed that the crude extract of OL has the highest zone of inhibition. The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%). However, the lowest yield (28.37%) was obtained from Oscpmum lamifolium (OL). Conclusion: Traditional Medicine is used by many people to managing numerous conditions; it’s accessible and effective on antimicrobial activity. Therefore, it plays a significant role by reducing life-threatening ailments of people and other animals.
Highlights
Learning via observation and experimentation indicates that plants have played as a major role for traditional medication and used to promote health of human beings
Different percentage yield of crude extract were obtained from Oscpmum lamifolium (OL), Croton maerosth (CM), and Ruta chalepesis (RC), selected medicinal plant
The highest yield of crude extract (38.21%) was obtained from Croton maerosth (CM) which followed by Ruta chalepesis (RC) (32.43%)
Summary
Learning via observation and experimentation indicates that plants have played as a major role for traditional medication and used to promote health of human beings. Traditional medicinal plants are used as remedies of safe for health, cost effective, almost free from serious side effect and used as health practice, approaches, knowledge and beliefs They are obtained from plant, animal and mineral based which used for spiritual therapies, being the plants are the most important source of medication. They used to apply in single or in combination to treat, diagnose and prevent illness and maintain well-being (WHO, 2001; Mazid, et al, 2012). Definition of traditional medicine by WHO as diverse health practices, approaches, knowledge and beliefs incorporating plant, animal, and/or mineral based medicines, spiritual therapies, manual techniques and exercises applied singularly or in combination to maintain well-being, as well as to treat, diagnose or prevent illness (WHO, 2002; 2000). Conducting the present study on the title of extraction and phytochemicals determination of traditional medicinal plants such as Ruta Chalepesis, Ocimum
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