Extraction and isolation of phlorotannins from brown seaweed Turbinaria ornata (turner) j. Agardh and its antioxidant activity

Abstract

The crude extract and fractions of Turbinaria ornata collected from Mandapam coast were evaluated for their antioxidant potentials. The antioxidant potential was determined using total antioxidant capacity, total phenolic content and DPPH radical scavenging activity. The ethyl acetate fraction obtained from the crude methanolic extract by different solvent extractions exhibited prominent antioxidant activity. The ethyl acetate fraction was further fractionated with column chromatography to yield 36 fractions. All the column purified fractions were subjected to thin layer chromatography analysis and the results produce 8 fractions. The antioxidant activity of crude methanolic extract and sub fractions were evaluated. The F5 fraction exhibited higher total phenolic content and antioxidant properties. On the basis of TLC, UV-Vis and FT-IR spectral analysis F5 fraction was found to contain phenolic compounds especially phlorotannins. To the best of our knowledge, the present study is the first report on the extraction and isolation of phlorotannins and this was responsible for antioxidant activity from brown seaweed Turbinaria ornata from India.