Extraction and isolation of alkaloids of Sophora alopecuroides and their anti-tumor effects in H22 tumor-bearing mice.

A Chang,Z Wang,S Sun,Z Cai

doi:10.4314/ajtcam.v11i2.3

Abstract

Alkaloids of Sophora alopecuroides have good biological activity, and are widely used in clinical settings, which not only have pharmacological activities of anti-cancer, cancer suppression, as well as the inhibition, and killing of various microorganisms; but also possess extensive pharmacological effects on immune system, nervous system and cardiovascular system. The objective of this paper was to extract and isolate total alkaloids of Sophora alopecuroides (TASA), and to study their anti-tumor effects in H22 tumor-bearing mice. TASA were extracted and isolated using thin-layer chromatography, and column chromatography; and the isolated compounds were analyzed using nuclear magnetic resonance. The inhibitory effects of TASA on tumor in H22-bearing mice were determined by MTT assay. Three compounds were isolated from Sophora alopecuroides L., which were matrine, oxymatrine and sophoridine, respectively. Meanwhile, mouse H22 sarcoma model was established and different doses of TASA apparently inhibited solid H22-tumor in mice; it inhibited the thymus, and spleen to some extent; the degree of inhibition was more obvious for the spleen. TASA has an anti-tumor effect in H22 tumor-bearing mice.

Highlights

Chinese medicine Ku Dou Zi (Sophora alopecuroides L.) is a plant in the genus Sophora, of family Fabaceae; named Ku Shen Cao, Ku Dou Gen, Ku Gan Cao, Xi Dou Gen, Ku Dou Cao, Ou Ku Shen, etc
The alkaloid chemical constituents in the seed of Sophora alopecuroides L. were studied; three compounds were isolated from the seed, which were matrine, oxymatrine and sophoridine
5kg, of Sophora alopecuroides L. coarse powder (10-20 mesh), was weighed, added with a 4-fold volume of 5% NaOH solution, and soaked overnight, the alkaline liquid was discarded, and the residue was further extracted with 5% sulfuric acid solution, the extract was passed through a cat-ion exchange resin; eluent was 95%, ethanol containing 3% ammonia, the eluents were combined, ethanol was recovered, after activated carbon adsorption, 312g of total alkaloids were obtained, which were isolated by a neutral alumina column, gradient eluted with petroleum ether-acetone to give various elution fractions

Summary

Introduction

Chinese medicine Ku Dou Zi (Sophora alopecuroides L.) is a plant in the genus Sophora, of family Fabaceae; named Ku Shen Cao, Ku Dou Gen, Ku Gan Cao, Xi Dou Gen, Ku Dou Cao, Ou Ku Shen, etc It is bitter in taste, cold in nature, and has the heat clearing, detoxifying, and pathogenic wind dispelling; dampness removing, analgesic, insecticidal, anti-bacterial as well as anti-inflammatory effects (Yang et al, 1998; Ren., 2000). Alkaloids of Sophora alopecuroides have good biological activity, and are widely used in clinical settings, which have pharmacological activities of anti-cancer, cancer suppression, as well as the inhibition, and killing of various microorganisms; and possess extensive pharmacological effects on immune system, nervous system and cardiovascular system. Conclusion: TASA has an anti-tumor effect in H22 tumor-bearing mice

Objectives

Methods

Conclusion