Abstract
Abstract The standard ethanol sugar extraction method and a chloroform-methanol-ethanol (CHCl3-MeOH-EtOH) extraction were compared. Determinations of sugar tri-methylsilyl derivatives by gas-liquid chromatography were examined. The CHCl3-MeOH-EtOH extraction was superior to the ethanol extraction. Complete recoveries of glucose were afforded by both methods; however, the ethanol extraction yielded maltose whereas the CHCl3-MeOH-EtOH method did not. Deionization of sweet potato sugar extracts and authentic sugars with MB-3 ion exchange resin was undesirable because only small percentages of sugars were recovered. Discrepancies were found in the recently published determination of sugar trimethylsilyl (TMS) derivatives by gas-liquid chromatography. Although it was reported that the quantity of the TMS product formed was essentially independent of time, at least 6 hr were necessary to completely form the penta-o-trimethylsilyl derivatives of fructose. The penta-o-trimethylsilyl derivatives of glucose were formed within 5 min as indicated in the literature. Anomerization from α- to β-fructose occurred after the introduction of the TMS reactants.
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