Extraction and Formulation of hydrolyzed-wool keratin solution for hair growth

Abstract

Hair growth is controlled by hair Follicles which show patterns of cyclic activity with periods of active growth and hair production (anagen), apoptosis-driven involution (catagen) and relative resting (telogen). These transformations are regulated by different variations like endocrine, vascular, neural, age and nutritional. Keratin biomaterial has been used in regenerative medicine owing to its in-vivo and in-vitro biocompatibility. The present study aimed at extracting keratin from wool by sulfitolysis, filtration by dialysis tube and freeze drying. Qualitative analysis of the keratin extract powder ensured the presence of cysteine, Keratin powder formulated as solution dosage form and pharmaceutically evaluated, Keratin extract was topically applied on the synchronized depilated dorsal skin of telogenic 4 mice and promoted hair growth by changing Hair growth initiation and completion time and quality of hair result, in wool keratin extract solution-treated groups hair growth initiation and completion time reduced and the hair result was hard, long, thick and healthy. Besides, that the keratin extract solution was a safe agent for topical administration, our study demonstrated that keratin extract stimulating hair growth by inducing the growth phase. The topical application of keratin extract may represent a promising biomaterial for the management and applications of hair follicle disorder.