Extraction and Determination of Evodiamine from Euodia Fructus with SPE-HPLC Based on a Homemade Phenyl-Based Monolithic Cartridge.

Abstract

A phenyl-based monolithic adsorbent was prepared in a 50-mm-long stainless steel tube, which was initiated by the redox system, using ethylene glycol phenyl ether acrylate as the monomer and ethylene glycol dimethacrylate as the crosslinker. The effects of monomer/crosslinker ratio and the porogens on the permeability and morphology of the resulting adsorbents were investigated, and the optimal adsorbent shows relatively uniform pore structure according to the characterizations of scanning electron microscopy and nitrogen adsorption-desorption method. The column that filled with the adsorbent was used as the solid-phase extraction (SPE) cartridge, exhibiting unique selectivity for the extraction of evodiamine from Euodia fructus (the fruits of Euodia rutaecarpa (Juss.)Benth.), which attributes to the interactions of π-π and hydrogen bonding between the adsorbent and evodiamine. Combined with a C18 analytical column via high-performance liquid chromatography (HPLC) system, an online SPE-HPLC method was established for extraction, enrichment and determination of evodiamine from Euodia fructus. Method validation demonstrates that the relative standard deviation of the precision is less than 0.66%, and the spiked recovery is in the range of 93.11-98.06%. Furthermore, it is worth noting that the prepared SPE cartridge can be reused for no less than 100 times. These results show that the developed method is simple and efficient for online extraction and enrichment of evodiamine from Euodia fructus.