Extraction and Comparison of Anthocyanin Content in Seeds of Different Black Rice Varieties Using Water Bath Solution Extraction Method

Abstract

Black rice, rare colored rice, is rich in anthocyanins. Black rice anthocyanin is a kind of natural plant pigment, which has the important effects of anti-oxidation, anti-inflammation and anti-cancer, protecting eyesight and promoting cardiovascular health. Fifteen representative black rice seeds in Southern Henan were used as test materials, and were determined and analyzed by using rice huller, visible light spectrophotometer and water bath solution extraction method and microwave assisted extraction method. The results showed that the anthocyanin content of different varieties of black rice varies greatly, among which the anthocyanin content of black fragrant glutinous rice No. 2 was the highest (0.4258%), and that of black fragrant glutinous rice No.4 was the lowest (0.0207%). The average content of anthocyanin in seeds of black rice varieties tested in Southern Henan Province was 0.1925%. Therefore, the above research results provide important clues for the genetic breeding and improvement of high-quality black rice varieties in Southern Henan. At the same time, varieties with higher anthocyanin content in black rice can be considered as backbone parents for new breeding high-quality black rice.