Abstract
Crepidium acuminatum (D. Don) Szlach (Syn. Malaxis acuminata, Trade Name: Jeevak) is an amazing nutraceutical orchid. In the ayurveda sector, it is a well-known herb because of its immense therapeutic potential. Pseudobulbs are the most in-demand component in the industry, out of all the segments of the plant. The activity is connected to the presence of several active metabolites. There are only a few research papers on C. acuminatum secondary metabolite extraction and characterization. We extracted and characterized β-sitosterol, Stigmasterol, and Eugenol from the pseudobulbs of this plant by High Performance Thin Layer Chromatography (HPTLC), Column Chromatography and High Performance Liquid Chromatography (HPLC). Nuclear Magnetic Resonance (NMR), Mass Spectroscopy (MS), Ultra Violet (UV), and Fourier Transform-Infrared (FT-IR) spectroscopy were utilised to characterise compounds. HPLC analysis revealed 99.98 μg/ml of β-sitosterol in the sample. Eugenol, Stigmasterol, and β-sitosterol, metabolites have a high therapeutic potential, indicating that they will be important medications in the future. Clinical trials are needed to confirm their effects on life-threatening disorders. The project's purpose is to inspire other scientists to launch a series of scientific inquiries into Crepidium acuminatum (Malaxis acuminata), and other orchids to fill in the gaps in the knowledge.
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