Abstract
Lectins are proteins essential for the biological recognition of cells a key factor for killing microbes. Since, legumes are good source, we extracted lectin from Cicerarietinum Black (CiarBL), Prunusdulcis nut raw (PruDuNRL), characterized for its key properties. The protein content of CiarBL was 13.13 ± 4.53, 25.45 ± 3.93, specific activity was 472.97, 243.80HAU/mg protein and for Prunusdulcis nut raw it was 04.60 ± 1.60, 17.84 ± 1.41and specific activity was 365.71, 252.52 HAU/mg protein. The total hemeagglutination activity of both lectin was found to be 512HAU. Blood group specificity analysis showed specificity to O+ and B+ for CiarBL, and for PruDuNRL O+, A+, B+ human erythrocytes both the lectin was specific to goat blood among the animal blood tested. The hemeagglutination inhibition assay showed specificity to glucose, maltose alone for CiarBL. While, PruDuNRL was glucose, sucrose, maltose, xylose specific. Both exhibited good antioxidant activity but erythrocyte membrane stability was higher with CiarBL when compared to PruDuNRL. CiarBL demonstrated promising stability to urea. While, PruDuNRL depicted sensitivity to pH, temperature, urea but both showed stability to salts such as FeCl3, Na2So4, CaCl2. SDS PAGE, authenticate it as low molecular weight i.e. 20, 29 KDa for CiarBL, for PruDuNRL 20 to 60KDa, 115KDa, the molecular weight was ranging from low to high. The antibacterial effect against gram positive oral pathogenic bacteria showed good result for CiarBL against Streptococcus oralis, Streptococcus pyogens at all concentration studied, moderate activity with Propionibacterium acnes, Staphylococcus aureus at 100 µg/ml. For PruDuNRL, the antibacterial activity was found to be decreased in the order of Streptococcus pyogenes < Streptococcus oralis < Propionibacterium acnes < Staphylococcus aureus. The possible mechanism involved was lectin interaction with teichoic and teichuronic acids, peptidoglycans, lipopolysaccharides on the surface of the bacteria. Lectin possessing good antioxidant, anti-inflammatory activity could hold better pharmacological applications. So, the result obtained from the present study paves way for further research in various avenues of clinical microbiology.
Published Version
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