Abstract
Organisms are constantly bombarded with DNA damaging factors from sources both within and outside their cells. In response, complex repair pathways have evolved to handle all types of DNA damage, such as double and single strand breaks and base oxidation. Different pathways invoke a variety of signaling and repair enzymes, and accurate measurement of the recruitment timescales and interplay between repair protein kinetics is paramount to mapping these damage responses. One popular method to this end is laser microirradiation.
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