Extracting female inbred lines from commercial sunflower hybrids

Claudio Guilherme Portela De Carvalho,José Francisco Ferraz De Toledo

doi:10.1590/s0100-204x2008000900009

Claudio Guilherme Portela De Carvalho, José Francisco Ferraz De Toledo

Open Access

https://doi.org/10.1590/s0100-204x2008000900009

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Abstract

The objective of this study was to obtain female inbred lines from sunflower (Helianthus annuus) hybrids. A methodology based on altering inbred lines carrying the fertility restorer gene (Rf) obtained from self pollinating hybrids into inbred lines with normal cytoplasm without the Rf gene was described. Further, derived male-sterile inbred lines were developed. The methodology was successfully used to obtain female inbreds from sunflower commercial hybrids. Although more time and labor consuming than the conventional female inbred line extraction methods, this methodology is advantageous in exploiting superior germplasms (commercial hybrids), which prompted us to develop practical procedures to allow its routinely use.

Highlights

Comercial sunflower (Helianthus annuus L.) hybrid production usually relies on cytoplasmic male sterility (CMS) associated to a genetic system for fertility restoration (Miller & Fick, 1997)
Cytoplasmic male-sterile inbred lines from all RHA inbreds included in this study were obtained using the proposed methodology
Only genotypes expressing monogenic fertility restoration for the CMS PET1 were used, because in more than 40 CMS sources identified since the discovery of the CMS PET1, by Leclercq in 1968, from a cross between H. petiolaris and H. annuus, fertility restoration has been found to be predominantly controlled by either one or two genes (Serieys, 1996)

Summary

Introduction

Comercial sunflower (Helianthus annuus L.) hybrid production usually relies on cytoplasmic male sterility (CMS) associated to a genetic system for fertility restoration (Miller & Fick, 1997). In this process, the female parental inbred line is a CMS-HA, which carries the male-sterile cytoplasm trait (S-type cytoplasm) but no fertility restorer gene (Rf) in the nucleus. The female parent is, a male-sterile inbred line with S-rfrf genotype. The male parental line is a RHA inbred line, which can carry a normal (N) or S-type cytoplasm, but must necessarily carry the Rf gene. The RHA inbred is male-fertile with either S-RfRf or N-RfRf genotype. The resulting hybrid is male-fertile with S-Rfrf genotype

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