Abstract
We discuss the use of an un-modified differential interference contrast microscope for characterization of the scatter and absorption properties of thin tissue samples. A simple calibration process is discussed and a phase-stepping procedure it demonstrated for acquiring phase information exclusive of absorption effects. The procedure results in two-dimensional displays of the local polar and azimuthal ray deviation. Alternatively, we show that these angular deviation maps can be integrated to provide estimates of the phase. We demonstrate the calibration process, illustrate a couple phase-stepping approaches, and show representative results for angular deviation and phase. Such results are useful in implementing Monte Carlo methods of propagating partial coherence within highly scattering media.
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