Abstract
Mast cells are effector cells that initiate allergic inflammatory immune responses by inducing inflammatory mediators. Boehmeria nivea (Linn.) Gaudich is a natural herb in the nettle family Urticaceae that possesses numerous pharmacological properties. Despite the various pharmacological benefits of Boehmeria nivea, its effects on allergic inflammation have not yet been determined. Here, we investigated the effect of the ethanol extract of Boehmeria nivea (BNE) on degranulation rat basophilic leukemia (RBL)-2H3 mast cells stimulated with anti-dinitrophenyl (anti-DNP) and bovine serum albumin (BSA) during immunoglobulin E (IgE)-mediated allergic immune response. The results showed inhibition of the release of β-hexosaminidase and histamine from the cells. BNE suppressed pro-inflammatory cytokines (Tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, and IL-6) and reduced T helper (Th)2 cytokine IL-4 expression and/or secretion correlated with the downregulation of p38, extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) signaling pathways in treated RBL-2H3 mast cells. In passive cutaneous anaphylaxis, treatment with BNE during IgE-mediated local allergic reaction triggered a reduction in mouse ear pigmentation and thickness. Taken together, these results indicated that BNE suppressed mast cell-mediated inflammation, suggesting that BNE might be a candidate for the treatment of various allergic disorders.
Highlights
Mast cells have been recognized to be involved in various types of allergic reactions including inflammatory disorders [1]
To study the potential regulatory role of Boehmeria nivea (BNE) in allergic reactions and as a treatment for allergic disorders, we identified the phenolic content and major ingredients of BNE using high performance liquid chromatography (HPLC) chromatograms
The results of the HPLC profile of BNE indicated that rutin, luteorin-7-glucoside, naringin, and hesperidin were the major phenolic components of BNE (Figure 1A)
Summary
Mast cells have been recognized to be involved in various types of allergic reactions including inflammatory disorders [1]. Molecules 2020, 25, 4178 cytokines (Tumor necrosis factor (TNF)-α, Interleukin (IL)-1, IL-6, and T helper (Th)2) that contribute to the initiation of allergic reactions by recruiting and activating eosinophils, neutrophils, and Th2 cells, as well as interacting with tissue cells [3]. These mediators are associated with risk factor responses in allergic diseases that result from widespread mast cell activation, which may lead to the potentially life-threatening outcome of anaphylaxis [2,4]. An effective and a safe treatment remains a medical need
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