Extract ethanol kelor (Moringa oleifera L.) leaves can reduce liver tissues damage Wistar white rat given 30% ethanol

Abstract

The ethanol extract of kelor (Moringa oleifera L.) leaves is thought to confer hepatoprotective effect. This study aimed to prove that administration of ethanol extract of kelor had increased glutathione peroxide (GPx) activity and decreased levels of malondialdehyde (MDA) of liver tissuess, characterized by decreased expression of intercellular adhesion molecule-1 (ICAM-1), steatosis, as well as aspartate aminotransferase (AST) activity. This randomized posttest control group design utilized 32 male Wistar rats that were equally divided into 4 treatment groups viz. K-(negative control), K+ (positive control, 1 mL of 30% ethanol/200 g body weight/day for the first 4 weeks of the experiment), P1 (1 ml ethanol 30%/200g BW/ day for 28th then starting on the 29th day was given M. oleifera L. extract of 5 mL /kg BW/day), P2 (given M. oleifera L. extract 5 mL/kg BW/day then the next 2 hours were given 1 ml of ethanol 30%/200 g body weight/day for 8 weeks (56 days). Data analysis was carried out with the Kruskal Wallis and Mann Whitney Tests. The results showed that the ethanol extract of M. oleifera L. could suppress liver tissues damage of Wistar white rat given 30 % ethanol by reducing oxidative stress which was characterized by increased GPx activity and decreased MDA levels in liver tissues as well as serum ALT and AST activity (P<0.01), and the highest expression of ICAM−1 in liver tissues and serum ALT and AST activity (p<0.05) and the highest average steatosis cells found in K+ and the lowest was in P2, MDA levels, ALT and AST activity (p<0.05) and the high average steatosis cells found in K+ and lower in P1 and P2. The means cell statistic was higher in P2 compared to P1. Likewise, the highest average cell necrosis was found in K+ and the lowest P2. MDA levels, ALT and AST activity were lower in the group given M. oleifera L. extract ethanol P2 compared to P1. The conclusions of the study are that M. oleifera L. can reduce liver tissues damage by reducing oxidative stress through increasing GPx activity, decreasing MDA levels in liver tissues, ALT and AST activity of Wistar rat fed 30 % ethanol.