Abstract
A comparison of extra-column effects of chromatographic peaks in high-performance liquid chromatography recorded using a flow-through polarographic detector and a typical UV (254 nm) detector is presented. The diameter of the detection channel of the polarographic detector was 2 mm and the volume of the detection channel of the UV detector was 9.2 μl. When extra-column effects due to injection, tubing and the electronic system were considered, it was found that within experimental error the values of the peak variances are comparable for UV and polarographic detection and equal to (2.7 ± 1.0)·103 μl2.
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