Abstract
Two full length copies of Cassava latent virus (CLV) DNA1 were cloned in head to tail arrangement on a plant expression vector to evaluate the potential of CLV for the development of an extrachromosomal vector system in plants. After direct transfer of the plasmid into protoplasts of Nicotiana tabacum cv. Petit Havana SR1 extrachromosomal single-stranded (ss) and double-stranded (ds) forms of DNA1 appeared after the first cell division of protoplasts. The extrachromosomal copies could also be detected within transformants which has been regenerated from kanamycin-resistant calli. The CLV-harbouring transformants do not display any symptoms usually observed after CLV infection. Stable conservation of extrachromosomal DNA1 was observed in F1 plants derived from self-pollination and in plants regenerated from protoplasts of transformants. Our data show that dimer constructs of CLV DNA1 are attractive candidates for an extrachromosomal plant vector system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
