Abstract
BPV 1-induced hamster tumors were used as model system to investigate the state of viral DNA in non-virus-producing tumors arising from papillomavirus infection. DNAs from a fibroma and three fibrosarcomas were analyzed for BPV 1 DNA by the Southern blot technique. The vast majority of viral DNA persists in a nonintegrated form, since: (i) supercoiled BPV-DNA can be purified by CsCl-ethidium bromide equilibrium centrifugation, (ii) restriction endonucleases, which do not cut viral DNA, do not change the DNA pattern in comparison to uncleaved samples, and (iii) digests of BPV-DNA with restriction enzymes HpaI, HpaII, and HhaI, respectively, lead to identical patterns with DNA from hamster tumors and from warts. Less than one genome equivalent per cell could persist in an integrated form. Virus-specific, high-molecular-weight DNA in the tumors may be interpreted as oligomeric or catenated DNA. Cleavage analysis of tumor DNA with the restriction enzymes HpaII and HhaI, both sensitive to DNA methylation, provided no evidence for methylation of BPV 1 DNA in hamster tumors.
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