Abstract
The present study focuses on the microbial synthesis of zinc oxide nanoparticles (ZnO NPs) and evaluating the antimicrobial property on foodborne pathogens. The bacterial strain, Acinetobacter schindleri SIZ7 was isolated from the waste filling area of Sivakasi, Tamil Nadu, India. The biogenic synthesis of ZnO NPs was carried out at room temperature and under suitable, eco-friendly environment using culture supernatant of A. schindleri. The physico-chemical properties exhibited by the biogenic ZnO NPs were characterised using UV-Visible Spectrophotometry, Energy dispersive X-ray spectroscopy (EDS), High Resolution Transmission Electron Microscopy (HRTEM), Fourier Transformed Infrared spectroscopy (FTIR) and Thermogravitometric Analysis (TGA). The synthesized ZnO NPs are polydispersed and spherical in shape. The antimicrobial activity of ZnO NPs was investigated against foodborne pathogens, Staphylococcus aureus (MTCC 96), Escherichia coli (MTCC 739), Vibrio parahaemolyticus (MTCC 451) and Salmonella enterica (MTCC 9844). The prepared ZnO NPs exhibited strong antimicrobial activity against E. coli and S. enterica with a minimum inhibitory concentration of 100µg ml-1. Thus, the bacterial strain Acinetobacter schindleri SIZ7 could be used for simple, extracellular, non-hazardous and efficient synthesis of antimicrobial ZnO NPs.
Highlights
Metallic nanoparticles are receiving considerable attention in agriculture and medicine due to their unique physical and chemical properties
The analysis of scanning electron microscope (SEM) photographs revealed that A. schindleri SIZ7 was rod shaped bacteria (Fig. 2)
Extracellular biosynthesis of Zinc oxide nanoparticles (ZnO NPs) was observed by visual colour change, pale yellow to fluorescent yellow after 48 h of incubation (Fig. 3)
Summary
Metallic nanoparticles are receiving considerable attention in agriculture and medicine due to their unique physical and chemical properties. Metallic NPs synthesis through bacteria, yeast, fungi, plant biomass, live plants, and plant extracts offer several advantages than chemical and physical route of synthesis (Castro-Longoria et al, 2011). Researchers reported the synthesized silver and gold nanoparticles using bacteria. Zinc oxide nanoparticles (ZnO NPs) have gained much interest owing to their wide applications in the preparation of personal care products, coatings and catalysts in environmental remediation, as antifungal and antibacterial agents (Kirthi et al, 2011). Jayaseelan et al, (2012) reported the bacterial mediated synthesis of ZnO NPs using Aeromonas hydrophila and its antimicrobial activity was established. Various researchers reported the antibacterial and antifungal properties of ZnO NPs (Tayel et al, 2011 and Xie et al, 2011). Our aim in the present study was to synthesize ZnO NPs using Acinetobacter schindleri SIZ7 and to investigate their antibacterial activity against foodborne pathogens
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