Abstract
Extract of oven dried leaves of Pongamia pinnata (L) Pierre was used for the synthesis of silver nanoparticles. Stable and crystalline silver nanoparticles were formed by the treatment of aqueous solution of AgNO3 (1mM) with dried leaf extract of Pongamia pinnata (L) Pierre. UV-visible spectroscopy studies were carried out to quantify the formation of silver nanoparticles. Transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy were used to characterize the silver nanoparticles. TEM image divulges that silver nanoparticles are quite polydispersed, the size ranging from 20 nm to 50 nm with an average of 38 nm. Water soluble heterocyclic compounds such as flavones were mainly responsible for the reduction and stabilization of the nanoparticles. Silver nanoparticles were effective against Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538p), Pseudomonas aeruginosa (ATCC 9027) and Klebsiella pneumoniae (clinical isolate). The move towards extracellular synthesis using dried biomass appears to be cost effective, eco-friendly to the conventional methods of nanoparticles synthesis.
Highlights
Nanobiotechnology, a branch of Nanoscience has been playing a decisive role in 21st century in deciphering diverse tribulations in the field of farming, medication and electronics
The present decade has witnessed the rapid shift in synthesis strategies from physicochemical methods to biological agents such as bacteria, fungi and plants for nanoparticle synthesis [2,3,4]
The antibacterial activity of silver nanoparticles was studied against Staphylococcus aureus (ATCC 6538P), Escherichia coli (ATCC 8739), Pseudomonas aeruginosa
Summary
Preparation of dried biomass The twigs containing mature leaves of Pongamia pinnata (L.) Pierre were collected from University of Mumbai campus. Accurately weighed dried leaf biomass of Pongamia (0.1 g, 0.5 g, and 1 g) was added to the sterile 50 mL 1mM aqueous AgNO3 [Merck] solution in Dippy’s jar of 250 mL under aseptic conditions. XRD measurements of purified silver nanoparticle solution casted onto the glass substrate was carried out using Phillips PW 1830 instrument operating at a voltage of 40 kV and current of 20 Ma with Cu K (D) radiation of 1.54187 nm wavelength. The sample purified as stated in XRD measurement section was sonicated (Vibronics VS 80) for 5 minutes. The FTIR measurements were carried out for both the dried biomass of Pongamia pinnata leaves and washed silver nanoparticle solution, free from any biomass residue or compound except the capping ligand using Perkin Elmer (Spectrum One) spectrophotometer
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