Abstract

The ability of an actinomycete, isolated from peat (labelled A IV 17), to produce extracellular protease in various low-cost media has been examined. It has been found that for protease production with the strain A IV 17 glucose can be replaced as energy source by xylose or glycerol. Horn meal, representing a cheap slaughtery waste product, has been found to be a favourable nitrogen source for the production of A IV 17-protease. With small changes in the composition of the medium, used for protease production, the productivity of the organism in question has been more than doubled, as compared with the protease yields achieved in the skim-milk medium, originally used for the screening of extracellular proteases produced by actinomycetes, isolated from soil. Die Fähigkeit eines Actinomyceten (A IV 17 genannt), der von Torf isoliert worden war, extrazellulare Protease in verschiedenen billigen Medien zu produzieren, wurde erforscht. Als Energiequelle für die Proteaseproduktion mit Stamm A IV 17 kann Glukose durch Xylose oder Glyzerin ersetzt werden. Hornmehl, das einen billigen Schlachtabfall darstellt, hat sich als eine beachtenswerte Stickstoffquelle in der Produktion von A IV 17- Protease erwiesen. Mit einigen kleinen Veränderungen in der Zusammensetzung des Produktionmediums wurde die Enzymerzeugung mehr als verdoppelt im Vergleich mit der im Milchpulvermedium (ohne Fett), das ursprünglich für den Vergleich der Produktion extrazellularer Proteasen verschiedener Boden-Actinomyceten benutzt worden war.

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