Abstract
In the present study, Penicillium purpurogenum BKS9 was used with different agro waste substrates i.e. starch, wheat bran, soya powder, boiled rice, unboiled rice and milk powder for the production of both amylase and protease by liquid static surface fermentation (LSSF). Among the various substrates tested, wheat bran (WB) was found to be the best substrate for maximum (112.64 U/ml) amylase production whereas soya powder (121.23 U/ml) for production of protease. Immobilization study also revealed that the highest amylase was observed (137.6 U/ml) when wheat bran was used as substrate whereas maximum protease production (130.73 U/ml) with soya seed powder. Maximum biomass production was observed (4.4 ± 0.2 g/50ml) when unboiled rice was taken as a substrate in comparison to other substrate.
Highlights
Among the various substrates tested for amylase activity, wheat bran was found to be the best substrate for maximum (112.64 U/ml) amylase production (Fig. 1)
Maximum biomass was obtained from P. purpurogenum when cultivated with rice (4.4 ± 0.2 g/50ml) at 30°C for 96 h (Fig. 2)
Bacterial amylases and proteases have long been used in industry, but, now-a-days, they are replaced by fungal amylases as these enzymes can be effortlessly extracted and separated from fungal biomass [16]
Summary
Among the various substrates tested for amylase activity, wheat bran was found to be the best substrate for maximum (112.64 U/ml) amylase production (Fig. 1). In case of production of protease soya powder (121.23 U/ml) was found to be the best substrate for P. purpurogenum (Fig. 1). Milk powder has a high potential as a substrate because both protease and amylase activities were found to be very high when used as a substrate
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