Abstract
The extracellular matrix (ECM) has a molecular machinery composed of diverse proteins and proteoforms that combine properties of tensile strength with extensibility exhibiting growth-regulatory functions and self- and non-self-recognition. The identification of ECM proteoforms is the prerequisite towards a comprehensive understanding of biological functions accomplished by the outermost layer of the cell. Regulatory mechanisms of protein functions rely on post-translational modifications, phosphorylation in particular, affecting enzymatic activity, interaction, localization and stability. To investigate the ECM proteoforms, we have isolated the cell wall proteome and phosphoproteome of a tuberous crop, potato (Solanum tuberosum). LC-MS/MS analysis led to the identification of 38 proteins and 35 phosphoproteins of known and unknown functions. The findings may provide a better understanding of biochemical machinery and the integrated protein and phosphoprotein network of ECM for future functional studies of different developmental pathways and guidance cues in mechanosensing and integrity signaling.
Highlights
Cellular homeostasis, semantics, and mechanical attributes are complex processes involving recruitment of cellular fibrils and differentiation of extracellular matrix (ECM)
The ECM-enriched pellet was washed repeatedly and extracted with low-osmolarity buffer to remove any contaminants from other organelles
The complete cell breakage was confirmed by analysis of isolated ECM fraction using a stereo zoom microscope
Summary
Semantics, and mechanical attributes are complex processes involving recruitment of cellular fibrils and differentiation of extracellular matrix (ECM). Cellular growth depends on the balance between mechanical force and turgor pressure mechanics This represents a nexus of various biological processes linking growth and development, plant-pathogen interactions, abiotic stress, self- and inter-organismal recognition, signaling components and metabolic processes. Proteomes 2016, 4, 20 multifunctional feature of ECM houses a compendium of dynamic, heterogeneous, and complex proteins and phosphoproteins. Reversible and sub-stoichiometric protein phosphorylation is an important posttranslational modification for cell-cell communication and signal transduction in cellular processes. Understanding ECM proteoforms might play major role in unraveling translational and post-translational information that modulates the biological processes and molecular functions in the outermost layer in potato. Protein and phosphoprotein network highlights that wall-associated signaling components, wall hydration, extension, deposition, and mechonosensing are the major functions of ECM in plant cells
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