Abstract
The extracellular matrix (ECM) was removed manually from the blastocoel of freeze-dried embryos of the newt, Cynops pyrrhogaster. In vitro examination demonstrated that the substratum of the blastocoelic ECM (B-ECM) promoted adhesion and spreading of dissociated gastrula cells, while little effect was shown on dissociated blastula cells under the same conditions. Furthermore, the B-ECM promoted locomotion of the spreading gastrula cells. SDS-polyacrylamide gel electrophoretic analysis revealed that the B-ECM contained three major proteins (108, 97 and 30 kilodaltons), which co-migrate with yolk proteins, and a 190-kilodalton protein. The yolk proteins extracted from yolk platelets were found also to act effectively as an strong adhesive sub-stratum for dissociated gastrula cells, however they did not promote cell locomotion. These results suggested that the yolk proteins in the B-ECM may act as an effective adhesion substance for dissociated gastrula cells.
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