Abstract
The localization of systemically induced chitinase activity was examined in the second leaves of cucumber plants that had been infected on the first leaf with tobacco necrosis virus. Leaf pieces were infiltrated with a buffered sodium chloride solution, and the intercellular fluid was subsequently extracted by centrifugation. Chitinase had a much higher specific activity in extracts of the intercellular fluid than in leaf homogenates. The specific activity of chitinase in protoplasts obtained from the leaves was only 5% of that in tissue homogenates. These data indicate that most of the chitinase activity is located in the extracellular space.
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