Extracellular glycoproteins of Candida bogoriensis

Abstract

1. 1. Extracellular glycoprotein was isolated from Candida bogoriensis. 2. 2. The glycoprotein was separated into two fractions by chromatography on Sephadex G-200. 3. 3. The fraction eluted first (L1) contained a greater proportion of carbohydrate and a smaller proportion of protein than the second fraction (L2). 4. 4. A high-molecular-weight fraction (H) was prepared by centrifuging the glyco-protein at 200 000 × g for 1.5 h. A cell extract was prepared by treating cells with 1 M NaOH. 5. 5. All the fractions and the extract contained fucose, rhamnose, mannose, galactose, glucose, and hexuronic acid. Mannose was the predominant sugar in Fraction L2 which contained very little fucose. Fraction L1 contained large amounts of all the sugars, especially fucose. Fraction H and the cell extract contained very little glucose. Fraction H contained more protein than Fraction L1 but less than Fraction L2. 6. 6. Amino acid analyses showed that Fraction L1 contained a large proportion of serine, glutamic acid and glycine. The other amino acids were present in approximately similar proportions to those of Fraction L2. 7. 7. The structural implications of these results are discussed.