Extracellular enzymes and βeta-lactamase screening of bacterial isolates cultured from urine samples of immunocompromised women

O V Olagoke,J O Jimoh,T O Ladipo,B I Alao

doi:10.30574/gscbps.2018.4.3.0043

Abstract

Enzymes intricate by organisms are used to inactivate and destroy antibiotics. This study determined some virulence factors elaborated by the bacterial isolates cultured from urine samples of HIV seropositive pregnant women that attended antenatal clinic of the Ondo State Specialist Hospital, Akure. Screening for extracellular enzymes and extended spectrum beta lactamase (ESBL) of the isolate was carried out using standard method and disk approximation method respectively. The extracellular enzymes such as lipase, protease and DNase were detected in the three bacterial genera used. ESBL was found in S. aureus and E. coli. The study concluded that most of the bacterial isolates cultured from urine samples of HIV seropositive pregnant women in the study area were found to have these virulence factors.

Highlights

Enzymes intricate by organisms are used to inactivate and destroy antibiotics
Determination of extra cellular enzymes production Determination of extra cellular enzymes production of bacterial isolates recovered from urine samples of HIV seropositive pregnant women were carried out for their ability to produce certain enzyme
Out of fifty bacterial isolates, fifteen of Staphylococcus aureus, one of Escherichia coli and five of Pseudomonas spp were positive for lipase

Summary

Introduction

Beta-lactamases are enzymes capable of hydrolyzing beta-lactam antimicrobials and at the same time inactivating them [1]. Extended-spectrum beta-lactamases (ESBLs) are beta-lactamase enzymes capable of hydrolyzing extended-spectrum or third generation cephalosporins (e.g., ceftriaxone and/or ceftazidime) [2]. They do not hydrolyze carbapenems and they are susceptible, in turn, to inhibition by conventional beta-lactamase inhibitors (clavulanate) [3]. ESBL producing organisms remain an important cause of therapy failure with beta-lactam antibiotics and have a serious impact on infection control [4]. The extended spectrum beta-lactamases (ESBLs) enable certain Gramnegative bacteria to inactivate cephalosporins as well as broad-spectrum penicillins and monobactams (aztreonam). ESBLs can present variations in the in vitro pattern of resistance to β-lactam agents conferred to the samples that encode them [9]

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