Extracellular Enzyme Activities in Relation to Hydrodynamics in the Pomeranian Bight (Southern Baltic Sea).

Abstract

Abstract The Pomeranian Bight in the southern Baltic Sea is characterized by a huge input of nutrients from the Oder river. This input shows seasonal variation. In winter, the nutrients are introduced in inorganic form. Particulate organic material is dominant in the growth season (summer/autumn). From 1993 to 1996, extracellular enzyme activities (alkaline phosphatase, peptidase, alpha-, beta-glucosidase, and chitinase) were investigated to describe microbial reaction to the input of organic material and the modification of introduced material. The distribution patterns of extracellular enzyme activities in salinity gradients were studied, in response to the nutrient load. These activities were distinctly lower in winter than in summer and autumn. A close relationship to other biological parameters (Chl a, POC, PON) was observed during the growth season, but not in winter. Changes in peptidase and phosphatase activities between summer and autumn were also observed. The peptidase activity was 9 to 72 times higher in autumn than in summer. In contrast, the alkaline phosphatase activity was 5 to 30 times higher in summer than in autumn. The organic compound turnover rate/hydrolysis rate (To/Hr) ratio is a relative index which describes the coupling of enzymatic hydrolysis and utilization of monomers from investigated substrates (carbohydrates and proteins). In summer, after dilution, the raised To/Hr quotients of glucose indicated limited importance for hydrolysis products in bacterial turnover. The increased demand for glucose resulted in a parallel decrease in monosaccharides. In autumn, the relationship between the turnover of glucose and amino acids and the supply of these substances by enzymatic degradation remained at the same level.