Abstract
Aspergillus fumigatus G-13 has the potential to degrade lignocellulose biomass. The purpose of this work is to analyze the extracellular soluble secretory protein of lignocellulose degradation by Aspergillus fumigatus G-13. The research used ferulic acid, sinapic acid and p-coumaric acid as carbon sources. By controlling the culture conditions, adding cellulose co-substrate and auxiliary carbon source, the enzymatic production law of Aspergillus fumigatus G-13 degradation of three lignin model compounds was investigated. The two groups with the greatest difference in enzyme activity expression were screened, and high throughput quantitative proteomics analysis using iTRAQ. iTRAQ analysis showed that a total of 3862 protein spots changed significantly, of which 2103 down-regulated proteins and 1759 up-regulated proteins. The differential proteins involved in the degradation process of lignin model compounds are concentrated in dioxygenase, oxidoreductase, ferulic acid esterase B-2, isoamyl alcohol oxidase, bifunctional catalase peroxidase CAT2, cellulase, cytochrome P450 monooxygenase, flavin-binding monooxygenase, etc. Lignin-related differential abundance proteins were mapped to 128 metabolic pathways. Significantly enriched pathways include metabolic pathways, glyceride metabolism, oxidative phosphorylation, riboflavin metabolism, peroxisomes, riboflavin metabolism. The information presented in this paper is helpful to better understand the lignocellulose degradation mechanisms of A. fumigatus G-13.
Highlights
Lignin is an amorphous aromatic polymer widely presented in plants, which contained a structural unit of oxyphenylene alcohol or a derivative thereof in its molecular structure (Tao & Guan, 2003)
The oblique surface of A. fumigatus G-13 PDA medium stored at 4 °C was taken out, and the fungal spores were washed with sterile water to prepare a spore suspension of 106 cells/mL
It can be seen that within 15 days of fermentation culture, the activities of three ligninases produced by A. fumigatus G-13 were all increased first, decreased, and eventually tended to be gentle, but the highest enzyme activity appeared at different time
Summary
Lignin is an amorphous aromatic polymer widely presented in plants, which contained a structural unit of oxyphenylene alcohol or a derivative thereof in its molecular structure (Tao & Guan, 2003). Most lignin is directly used as fuel or discharged into rivers, which wastes resources and causes environmental pollution (Himmel et al, 2007). The biodegradation of lignin has been a worldwide research focus and difficult problem. Clarifying the biodegradation mechanism of lignin is the primary key scientific issue which needs to be elucidated, and the urgent theoretical basis for solving the practical application problems. The study of lignin degradation mechanism needs to follow the change of substrate and start from enzyme itself. Proteomics is an effective means to explore protein action patterns, functional mechanisms, and regulation control as well as interrelationship within the
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
