Abstract
Extracellular Chromatin Traps Interconnect Cell Biology, Microbiology, and Immunology
Highlights
Goldmann and Medina (2013) introduce the topic of NETosis by describing aspects that differentiate NETosis from other forms of cell death and by reminding the readers that extracellular traps are produced by eosinophils, mast cells, and even monocytes/ macrophages in addition to neutrophils
Brinkmann et al (2013) present a semi-automated method for enumerating cells that traverse sequential stages in NETosis. This useful and adaptable method by the original discoverers of neutrophil extracellular traps (NETs) relies on dual channel fluorescence and compares binding of anti-chromatin antibodies relative to staining with a DNA-intercalating dye
The new method should be ideally suited to high throughput screening for drugs that affect the efficiency of NET release
Summary
Goldmann and Medina (2013) introduce the topic of NETosis by describing aspects that differentiate NETosis from other forms of cell death and by reminding the readers that extracellular traps are produced by eosinophils, mast cells, and even monocytes/ macrophages in addition to neutrophils. By reviewing PAD4 structure and function, the authors discuss inhibitors of PAD4 and their potential use in suppressing NETosis. Induced PAD4 expression leads to histone deimination and the release of NET-like webs from U2OS and NIH 3T3 cells.
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