Abstract
Thirteen species of Neisseria were examined for the presence of extracellular carbohydrate antigens. Of three media tested for optimum growth of the bacteria, yield of antigens, and ease of isolation of antigens, dialyzed tryptic soy broth was the best. Seven species, N. catarrhalis, N. cinerea, N. flava, N. flavescens, N. lactamicus, N. subflava, and N. winogradski, yielded extracellular antigens that were extractable into phenol and hence were probably proteinaceous. The remaining six species, N. canis, N. caviae, N. cuniculi, N. cuniculi subsp. gigantea, N. denitrificans, and N. sicca, yielded carbohydrate antigens that were purified by a sequence of phenol extraction, chromatography, enzymic digestion, and membrane filtration. These antigens were polymers of 2-acetamido-2-deoxy-D-glucose and 2-acetamido-2-deoxy-D-galactose, the latter being the only constituent of the antigens from N. caviae and N. sicca. Cross-reactivities of the carbohydrate antigens in the homologous antisera showed that there were close relations between the antigens from N. canis and N. cuniculi subsp. gigantea and also between those from N. caviae and N. sicca.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callSimilar Papers
More From: Canadian journal of microbiology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
