Abstract
We constructed a plant transformation intermediate vector pBZ3, which contains the RbcS-GUS chimeric gene and this was transferred into tobacco NC89 leaf discs. The RbcS promoter maintained photospecificity since we observed strong GUS-staining from light-grown transgenic seedlings while no visible GUS activity was found from etiolated seedlings. When purified CaM was injected into the hypocotyls of these etiolated seedlings, the promoter became functional even under complete darkness. Northern blot analysis showed that both green seedlings and CaM-treated etiolated seedlings contained significantly higher amount of GUS mRNA when compared to that of untreated etiolated seedlings. The level of active calmodulin in etiolated seedlings was found much lower than that in green seedlings as determined by Western blot analysis. Our results indicated that CaM is involved in photoregulated gene expression.
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