Extracellular Accumulation of Cyclic Adenosine 3', 5'-Monophosphate by Hydrocarbon-utilizing Bacteria

Abstract

During the growth of hydrocarbon-utilizing bacteria, Arthrobacter roseoparaffineus KY 4301 and Micrococcus paraffinoiyticus KY 4306 on n-alkane as the sole source of carbon, it was found that a considerable amount of an adenine ribonucleotide accumulated in the medium. The nucleotide was isolated in crystalline form from the broth culture through the chromatography on ion-exchange resins and Sephadex G–10 columns. It was identified as cyclic adenosine 3′,5′-monophosphate by paper chromatography, ultraviolet-absorption spectra, infrared-absorption spectra and other chemical analyses.For the production of cyclic adenosine 3′,5′-monophosphate, n-alkane, particularly n-dodecane or n-tetradecane, was the most preferable carbon source under the culture condition in this work. With n-tetradecane, approximately 1.4 g per liter and 0.5 g per liter were produced respectively by Arthrobacter roseoparaffineus KY 4301 and Micrococcus paraffinolyticus KY 4306 under the same condition.