Abstract

Extra Cellular Lipase Production, Purification and Characterization by Using Bacillus Subtilis in Submerged State Fermentation

Highlights

  • Lipase is produced by various microbes, bacteria, fungi, yeast, mammals and plants in large amounts

  • Extracellular lipase produced by microorganisms is being investigated for its potential application in various industrial processes like detergents, oils, fatty acids and diary coupled with enormous therapeutic uses [3]

  • The bacillus subtilis was used for the production of extracellular lipase by submerged state fermentation by using various substrates

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Summary

Introduction

Lipase is produced by various microbes, bacteria, fungi, yeast, mammals and plants in large amounts. Multi-faceted microbial lipases (tricyglycerol acylhydrolase, E.C.3.1.1.3) have emerged as key enzymes in swiftly growing modern biotechnology lipases are indispensable for the bioconversion lipids (triacylglycerols.) from one organism to another and within the organisms. In addition to their biological significance, lipase have tremendous potential in areas such as food technology, bio medical sciences and chemical industry, [1315]. Many different bacterial species produce lipases, which hydrolyze the esters of glycerol with preferably long chain fatty acids. They act at the interface generated by a hydrophobic liquid substrate in a hydrophilic aqueous medium [17]

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