Abstract
The Rotimer, a rotifer-specific biopolymer, is an exogenic bioactive exudate secreted by different monogonant species (e.g. Euchlanis dilatata or Lecane bulla). The production of this viscoelastic biomolecule is induced by different micro-particles, thereby forming a special Rotimer-Inductor Conglomerate (RIC) in a web format. In this case, the water insoluble Carmine crystals, filtered to size (max. diameter was 50 µm), functioned as an inductor. The RIC production is an adequate empirical indicator to follow up this filamentous biopolymer secretion experientially; moreover, this procedure is very sensitive to the environmental factors (temperature, pH, metals and possible natural pollutant agents). The above mentioned species show completely different reactions to these factors, except to the presence of calcium and to the modulating effects of different drugs. One of the novelties of this work is that the Rotimer secretion and consequently, the RIC-formation is a mutually obligatory and evolutionary calcium-dependent process in the concerned monogonants. This in vivo procedure needs calcium, both for the physiology of animals and for fiber formation, particularly in the latter case. The conglomerate covered area (%) and the detection of the longest filament (mm) of the given RIC were the generally and simultaneously applied methods in the current modulating experiments. Exploring the regulatory (e.g. calcium-dependency) and stimulating (e.g. Lucidril effect) possibilities of biopolymer secretion are the basis for optimizing the RIC-production capacities of these micro-metazoans.
Highlights
Nowadays, molecular investigations are dominant parts of biological research
The aim of this study is to investigate selected environmental factors and possible pollutant agents on Rotimer secretion, experientially monitored by Rotimer-Inductor Conglomerate (RIC) formation
The rotifers are validated models of toxicity screening related to environmental parameters and chemical agents (Rico-Martínez et al, 2016)
Summary
Molecular investigations are dominant parts of biological research. Despite of this, the renaissance of supramolecular and expe riential biology is manifested in the various applications of their numerous advantages. Rotifers highly tolerate natural environmental changes; they are able to survive lifeincompatible conditions by halting activities, such as reducing meta bolism by suspending active life or egg production (Jonsson and Wojcik, 2017; Kanazawa et al, 2017; Shain et al, 2016). Benefiting from these evolutionally developed anatomic and physiologic abilities (Gilbert, 2017), rotifers respond to external challenges by producing different types of biomolecules (e.g. biopolymers; Datki et al, 2021) applying optimized phenotypic plasticity
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