Abstract

The study were undertaken on 16 Iraqi Awassi ewes, presented in the farm of college of veterinary medicine, Al- Fallujah University/ Fallujah, during the end of February to the half of the march, 2018. The age of the animals ranged between 2 to 4 years. All the ewes were examined by ultrasonography by specialist in order to be sure that the animals not pregnant with B- mode system. All the animals has been synchronized with vaginal sponges impregnated in 40 mg flurogestone acetate (intervet, B.V. Boxemeer, Holland). The animals were put in a standing position clipping and shaving of the left flank area then the site washed with antiseptic and disinfected with tincture iodine. The animals anesthetized locally using 2% xylocaine hydrochloride using 10 ml subcutaneously and intra muscular. left flank sharp dissection about 10-12 cm were made through the skin, muscle and peritoneum. Exteriorization of the uterus and ovaries were done to detect the pregnancy through a symmetry of uterine horns and the enlargement of pregnant horn or to detect the numbers of follicles and corpora lutea after that the peritoneum and the muscles were sutured with catgut no.1 with lock stich pattern. The skin was sutured with silk no.1 using horizontal mattress interrupted pattern. Systemic long acting tetracycline were applied I.M (200 mg/ ml: 100mg/kg B.W) for one day and another for 3 injection. Local antibiotic spray was applied to treat the surgical wounds for 7 days. The suture materials of the skin were removed after 7 days of the operation. The animals were examined daily for general health condition (temperature, heart rate, pulse rate, appetite). The wound also examined for presence of swelling, hematoma, pus or infection or hernia Positive results were considered when the animal in a good general health condition and the wound showed normal healing without presence of swelling, hematoma, pus or infection or hernia. This technique is simple, easily done, save time and cost as well as reduce the surgical trauma with few suture materials. The study was the first of its kind all over the world in exteriorization of genital organs in standing position in ewes.

Highlights

  • Awassi ewes considered one of the most important breed in Iraq

  • The ewes injected with equine chorionic gonadotropine (500 I.U) I.M, when the sponges removed the animals were injected with human chorionic gonadotropine (HCG)

  • The animals divided into two groups, the first group were mated with a fertile ram when the ewe showed a sign of estrus, the animals followed for pregnancy detection with ultrasonography and laparotomy

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Summary

Introduction

Awassi ewes considered one of the most important breed in Iraq. It resist the high environmental temperature that leads to decrease their reproductive performance through the effects of temperatures on the uterus, hormones and fetus [2]. Awassi ewes may have a silent heat [3, 4]. Research to increase reproductive performance in awassi ewes including: superovulation, synchronization, pregnancy diagnosis, IVF and embryo transfer. Laparotomy was one of the diagnostic tools used for examination and exteriorization of the genital system of the ewes. There are several approach used for laparotomy in large animals including ventral, ventro-lateral, paramedian, mid-line and flank incision [5]. The aim of this study is to make laparotomy in standing position for Awassi sheep for superovulation, collection of oocytes and pregnancy diagnosis

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